na k atpase  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Low-load blood flow restriction training and ischemia modulate expression of Na + ,K + -ATPase and FXYDs in human skeletal muscle

doi: 10.64898/2025.12.24.696414

Figure Lengend Snippet: qPCR was used to determine gene expression of (A) NKAα1 ( ATP1A1 ), (B) NKAα2 ( ATP1A2 ), (C) NKAα3 ( ATP1A3 ), (D) NKAβ1 ( ATP1B1 ), (E) NKAβ2 ( ATP1B2 ), (F) NKAβ3 ( ATP1B3 ), (G) FXYD1 , and (H) FXYD5 . 18S rRNA was used as endogenous control. Immunoblotting was used to estimate protein abundance of (I) NKAα1, (J) NKAα2, (K) NKAβ1, (L) FXYD1, (M) FXYD5, and (N) Sp1. Results are means ± SD (n=3-6); * P <0.05 vs. Control; # P <0.05 vs LL-Sham. Kruskal-Wallis with Dunn‘s post-hoc test was used for statistical evaluation.

Article Snippet: Polyclonal antibodies against AMPKα (cs-2532) , monoclonal antibodies against phospho-AMPKα (Thr 172 ) (cs-2535) ( , , ), monoclonal antibodies against acetyl-CoA carboxylase (cs-3676) ( , ), polyclonal antibodies against phospho-acetyl-CoA carboxylase (Ser 79 ) (cs-3661) ( , ), monoclonal antibodies against phospho-NKAα1 (Tyr 10 ) (E1Y9C) (cs-13566) ( ) and monoclonal antibodies against Sp1 (D4C3) (cs-9389) ( ) were from Cell Signaling Technology.

Techniques: Gene Expression, Control, Western Blot, Quantitative Proteomics

Journal: bioRxiv

Article Title: Low-load blood flow restriction training and ischemia modulate expression of Na + ,K + -ATPase and FXYDs in human skeletal muscle

doi: 10.64898/2025.12.24.696414

Figure Lengend Snippet: For the last 24 hours differentiated human myotubes were exposed to normoxia (NOR), glucose deficiency (glucose-free medium, NGD), hypoxia (0.1% O 2 , HYP), or both – ischemia (HGD) in serum-free DMEM. See text for details. qPCR was used to estimate gene expression of (A) NKAα1 ( ATP1A1 ), (B) NKAα2 ( ATP1A2 ), (C) NKAα3 ( ATP1A3 ), (D) NKAβ1 ( ATP1B1 ), (E) NKAβ2 ( ATP1B2 ), (F) NKAβ3 ( ATP1B3 ), (G) FXYD1 , and (H) FXYD5 . Immunoblotting was used to estimate protein abundance of (I) NKAα1, (J) pNKAα1 (Tyr 10 ), (K) phosphorylation of NKAα1, relative to total protein, (L) NKAα2, (M) NKAβ1, (N) Sp1, and (O) FXYD5. Results are means ± SD for n=6 for qPCR and n=5-6 for immunoblotting; * P <0.05 vs. NOR. In case of unspecific bands arrowheads mark the specific bands that were used in densitometric analysis. Ordinary one-way ANOVA with Dunnett’s post-hoc test was used for statistical evaluation.

Article Snippet: Polyclonal antibodies against AMPKα (cs-2532) , monoclonal antibodies against phospho-AMPKα (Thr 172 ) (cs-2535) ( , , ), monoclonal antibodies against acetyl-CoA carboxylase (cs-3676) ( , ), polyclonal antibodies against phospho-acetyl-CoA carboxylase (Ser 79 ) (cs-3661) ( , ), monoclonal antibodies against phospho-NKAα1 (Tyr 10 ) (E1Y9C) (cs-13566) ( ) and monoclonal antibodies against Sp1 (D4C3) (cs-9389) ( ) were from Cell Signaling Technology.

Techniques: Gene Expression, Western Blot, Quantitative Proteomics, Phospho-proteomics

Journal: bioRxiv

Article Title: Low-load blood flow restriction training and ischemia modulate expression of Na + ,K + -ATPase and FXYDs in human skeletal muscle

doi: 10.64898/2025.12.24.696414

Figure Lengend Snippet: qPCR was used to estimate gene expression of (A) NKAα1 ( ATP1A1 ), (B) NKAα2 ( ATP1A2 ), (C) NKAα3 ( ATP1A3 ), (D) NKAβ1 ( ATP1B1 ), (E) NKAβ2 ( ATP1B2 ), (F) NKAβ3 ( ATP1B3 ), (G) FXYD1 , and (H) FXYD5 . Immunoblotting was used to estimate protein abundance of (I) NKAα1, (J) pNKAα1 (Tyr 10 ), (K) phosphorylation of NKAα1, relative to total protein, (L) NKAα2, (M) NKAβ1, and (N) FXYD5. Results are means ± SD for n=5; * P <0.05 vs. NOR (+2h NOR or 2h HGD); the line with # indicates P <0.05 for effect of 2-hour ischemia (HGD) vs. control conditions (+2h NOR). In case of unspecific bands arrowheads mark the specific bands that were used in densitometric analysis. For qPCR results ordinary one-way ANOVA with Dunnett’s post-hoc test was used, while regular two-way ANOVA with Dunnett’s post-hoc was used for immunoblotting results.

Article Snippet: Polyclonal antibodies against AMPKα (cs-2532) , monoclonal antibodies against phospho-AMPKα (Thr 172 ) (cs-2535) ( , , ), monoclonal antibodies against acetyl-CoA carboxylase (cs-3676) ( , ), polyclonal antibodies against phospho-acetyl-CoA carboxylase (Ser 79 ) (cs-3661) ( , ), monoclonal antibodies against phospho-NKAα1 (Tyr 10 ) (E1Y9C) (cs-13566) ( ) and monoclonal antibodies against Sp1 (D4C3) (cs-9389) ( ) were from Cell Signaling Technology.

Techniques: Gene Expression, Western Blot, Quantitative Proteomics, Phospho-proteomics, Control